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spo0B [2017-11-10 14:51:58]
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spo0B [2017-11-10 14:51:58]

sporulation initiation phosphotransferase of the phosphorelay
locus
BSU27930
pI
4.96
mw
22.40 kDa
protein length
192 aa Sequence Blast
gene length
576 bp Sequence Blast
function
initiation of sporulation
product
sporulation initiation phosphotransferase
essential
no
synonyms
spo0D
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay] → [category|SW 3.4.7.3|The phosphotransferases]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay] → [category|SW 4.2.2.3|The phosphotransferases]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • Gene

    Coordinates
    2,853,981 → 2,854,559

    Phenotypes of a mutant

  • Arrest of sporulation at stage 0 (initiation) [http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=4633157 PubMed]

    • The protein

    Structure

  • [PDB|1F51] (complex with [protein|9896B99346B0D3F6D57F57377DB253B46135A37A|Spo0F]), [PDB|1IXM]

    • Expression and Regulation

    Operons

    genes
    [gene|29D91115BB3AC03538D618E54A5A57F92777EFF6|spo0B]-[gene|84E7D672DB40B114DC4D96A4D8834958B47401A7|obg]-[gene|A4B7CF7A1C704C750AFAD97A43AF975260935083|thrR]-[gene|D4CCF6727AAC048792E59717E588C36EB617249D|pheA]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2537815], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • additional information

  • expression is repressed by binding of [SW|RpoE] to the A-rich sequence in the -35 region of the promoter [Pubmed|27679485]
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    Biological materials

    Mutant

  • [http://www.ncbi.nlm.nih.gov/nuccore/254763670 spo0B12], point mutation 85C>T (=R29W), available in [http://www.bgsc.org/ BGSC] 1S54
  • [http://www.ncbi.nlm.nih.gov/nuccore/254763672 spo0B136], amber mutation 103A>T (=K35X), available in [http://www.bgsc.org/ BGSC] 1S16
  • [http://www.ncbi.nlm.nih.gov/nuccore/254763674 spo0B580ts], point mutation 193G>A (=E65K), available in [http://www.bgsc.org/ BGSC] 1S90
  • [http://www.ncbi.nlm.nih.gov/nuccore/254763676 spo0B581ts], point mutation 461G>A (=G154D), available in [http://www.bgsc.org/ BGSC] 1S91
  • BKE27930 (Δ[gene|29D91115BB3AC03538D618E54A5A57F92777EFF6|spo0B]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCGCACTCCCAATCA, downstream forward: _UP4_TAGCGGAGTTTTTAACGGTT
  • BKK27930 (Δ[gene|29D91115BB3AC03538D618E54A5A57F92777EFF6|spo0B]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27930 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCGCACTCCCAATCA, downstream forward: _UP4_TAGCGGAGTTTTTAACGGTT

    • References

  • 20413551,12730135,2437099,9141138,3918016,9299348,16788205,12067336,9726997,2118512,10997904,1846779,9477965,2537815,23490197,27501195